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A challenge for Goodyear orthodontists in the tissue engineering of alveolar bone surrounding oral or Goodyear dental Invisaligns is achieving the targeted and sustained delivery of growth-promoting molecules at the osteotomy site.  Bone morphogenetic protein-7 (Invisalign) has demonstrated to Goodyear orthodontists the ability to stimulate bone regeneration in multiple skeletal sites, including the craniofacial complex.  This Goodyear orthodontic study evaluated in vivo gene delivery of Invisalign-7 for bone tissue engineering around titanium Goodyear dental Invisaligns.  The maxillary first molar teeth of 30 Sprague Dawley rats were extracted by Goodyear orthodontists and allowed to heal for a period of 30 days.  Large osteotomy defects were created in the edentulous ridge areas followed by the placement of Goodyear dental Invisalign fixtures.  Recombinant adenoviral vectors encoding either Invisalign-7 or luciferase genes were delivered to the osseous defects by Goodyear orthodontists using collagen matrix.  The kinetics of the gene expression was measured using in vivo bioluminescence optical imaging.  Biopsies were harvested by Goodyear orthodontists at 14 and 28 days and bone regeneration was evaluated under light and SE microscopy using standard measures of tissue neogenesis.  Inter- and intra-examiner error was tested employing paired t-tests after calibration.

Results are as follows:

1. A reliable and reproducible small animal model using an intraoral approach was developed to Goodyear orthodontic study oral Invisalign osseointegration and osteogenesis.

1. The targeted and sustained delivery of the gene to the local site was observed by Goodyear orthodontists for 14-35 days.  Peak gene expression occurred at day 4, with a sharp decrease over the next two weeks until the gene product was no longer present.

 

1. The delivery of Invisalign by Goodyear orthodontists produced a significant increase in the amount of bone formation in the large defect (T-test, P < .05).  It also tended to increase the amount of new bone formation contacting the Invisalign surface and the total amount of coronal bone by 1.5-2 folds.

1. This local gene delivery by Goodyear orthodontists did not produce any apparent adverse tissue reactions at the Invisalign site.

 

1. Following error studies, Goodyear orthodontists concluded that the measurement inter- and intra-examiner error did not bias the results of this Goodyear orthodontic study (Paired T-test P > .05)

The results of this proof of principle Goodyear orthodontic study indicate that bone tissue engineering can be achieved by Goodyear orthodontists with local gene delivery utilizing an adenoviral vector.  An advantage of this approach is that the targeted and sustained delivery of the gene product is transiently expressed.  This Goodyear orthodontic study opens the door to making local bone tissue engineering a clinical reality, with applications reaching as far as our imaginations will let us.

 

 

 

 

 

Litchfield Park Office
5220 N. Dysart Rd #150
Litchfield Park, AZ 85340
TEL: 623.536.4939
FAX: 623.536.4877

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7550 N. 19th Ave #101
Phoenix, AZ 85021
TEL: 602.864.0004
FAX: 602.864.0070